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Selective Detection of NADPH among Four Pyridine‐Nucleotide Cofactors by a Fluorescent Probe Based on Aggregation‐Induced Emission
Author(s) -
Noguchi Takao,
Dawn Arnab,
Yoshihara Daisuke,
Tsuchiya Youichi,
Yamamoto Tatsuhiro,
Shinkai Seiji
Publication year - 2013
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201300015
Subject(s) - fluorescence , cofactor , nad+ kinase , chemistry , nucleotide , photochemistry , pyridine , stereochemistry , biophysics , biochemistry , biology , enzyme , medicinal chemistry , physics , quantum mechanics , gene
A fluorescent sensor based on guanidinium‐tethered tetraphenylethene (TPE) has been investigated toward the differentiation of pyridine nucleotide cofactors (NAD + , NADH, NADP + , and NADPH). TPE selectively recognizes NADPH possessing the higher tetra‐anionic net‐charge, resulting in the steep “turn‐on” fluorescence increase. The comparative aggregation behaviors and fluorescence response studies of TPE on the four cofactors reveal that the critical aggregate concentration of TPE against NADPH correlates directly with the concentration threshold for the fluorescence response. These results establish that TPE can selectively differentiate NADPH over the other three cofactors by the steep aggregation‐induced fluorescence response accompanied by the high signal‐to‐background contrast.