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Polydiacetylene Liposome Microarray Toward Influenza A Virus Detection: Effect of Target Size on Turn‐On Signaling
Author(s) -
Seo Sungbaek,
Lee Jiseok,
Choi EunJin,
Kim EunJu,
Song JaeYoung,
Kim Jinsang
Publication year - 2013
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201200819
Subject(s) - liposome , virus , microarray , sensory system , peptide , influenza a virus , biophysics , dna microarray , chemistry , biology , virology , biochemistry , gene , gene expression , neuroscience
Target size effect on the sensory signaling intensity of polydiacetylene (PDA) liposome microarrays was systematically investigated. Influenza A virus M1 peptide and M1 antibody were selected as a probe–target pair. While red fluorescence from the PDA liposome microarrays was observed when the larger M1 antibody was used as a target, when the same M1 antibody was used as a probe to detect the smaller M1 peptide sensory signal did not appear. The results reveal that the intensity of the PDA sensory signal is mainly related to the steric repulsion between probe–target complexes not the strength of the probe–target binding force. Based on this finding, we devised a PDA sensory system that directly detects influenza A whole virus as a larger target, and confirmed the target size effect on the signaling efficiency of PDA.