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Ultrasensitive Detection of Proteins on Western Blots with Semiconducting Polymer Dots
Author(s) -
Ye Fangmao,
Smith Polina B.,
Wu Changfeng,
Chiu Daniel T.
Publication year - 2013
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201200809
Subject(s) - blot , detection limit , fluorescence , sodium dodecyl sulfate , gel electrophoresis , chemistry , polyacrylamide gel electrophoresis , polymer , polyacrylamide , electrophoresis , polyvinylidene fluoride , chromatography , microbiology and biotechnology , materials science , biochemistry , biology , optics , polymer chemistry , enzyme , physics , gene , organic chemistry
Ultrasensitive fluorescence imaging of proteins on western blots using a bright, compact, and orange‐emitting semiconducting polymer dot (CN‐PPV) is demonstrated. A detection limit at the single‐picogram level in dot blots is achieved; with conventional western blotting, 50 pg of transferrin and trypsin inhibitor after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and transfer onto a polyvinylidene fluoride membrane are detected. This method does not require any additional equipment or time compared with the conventional procedure with traditional fluorescent probes.