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A Generic Strategy for Co‐Presentation of Heparin‐Binding Growth Factors Based on CVD Polymerization
Author(s) -
Deng Xiaopei,
Lahann Joerg
Publication year - 2012
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201200343
Subject(s) - polymerization , polymer chemistry , heparin , polymer science , chemical engineering , chemistry , materials science , combinatorial chemistry , polymer , organic chemistry , biochemistry , engineering
A multifunctional copolymer with both aldehyde and alkyne groups is synthesized by chemical vapor deposition (CVD) for orthogonal co‐immobilization of biomolecules. Surface analytical methods including FTIR and XPS are used to confirm the surface modification. Heparin‐binding growth factors [basic fibroblast growth factor (bFGF) in this study] can be immobilized through interaction with heparin, which was covalently attached to the CVD surface through an aldehyde‐hydrazide reaction. In parallel, an alkyne–azide reaction is used to orthogonally co‐immobilize an adhesion peptide as the second biomolecule.