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Preparation, Cellular Internalization, and Biocompatibility of Highly Fluorescent PMMA Nanoparticles
Author(s) -
Vollrath Antje,
Pretzel David,
Pietsch Christian,
Perevyazko Igor,
Schubert Stephanie,
Pavlov George M.,
Schubert Ulrich S.
Publication year - 2012
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201200329
Subject(s) - biocompatibility , methacrylate , hemolysis , chemistry , particle size , centrifugation , monomer , methyl methacrylate , materials science , biophysics , polymer chemistry , chromatography , polymer , organic chemistry , immunology , biology
Methacrylate monomers were functionalized with a 4‐hydroxythiazole chromophore and copolymerized with methyl methacrylate via RAFT. Nanoparticles of 120 and 500 nm in size were prepared without using stabilizers/surfactants. For comparative studies, preparative ultracentrifugation was applied for the separation into small and large particle fractions. All suspensions were characterized by DLS, AUC, and SEM and tested regarding their stability during centrifugation and re‐suspension, autoclavation, and incubation in cell culture media. In vitro studies with mouse fibroblast cell line and differently sized NP showed a particle uptake into cells. Biocompatibility, non‐toxicity, and hemocompatibility were demonstrated using a XTT assay, a live/dead staining, and an erythrocyte aggregation and hemolysis assay.