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Live Monitoring of Cargo Release From Peptide‐Based Hybrid Nanocapsules Induced by Enzyme Cleavage
Author(s) -
Andrieu Julien,
Kotman Niklas,
Maier Matthias,
Mailänder Volker,
Strauss Wolfgang S. L.,
Weiss Clemens K.,
Landfester Katharina
Publication year - 2012
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201100729
Subject(s) - nanocapsules , cleavage (geology) , peptide , enzyme , chemistry , biophysics , nanotechnology , biochemistry , materials science , biology , nanoparticle , composite material , fracture (geology)
The miniemulsion process is used as a new route for the preparation of enzyme‐responsive nanocapsules with payload‐release properties. Peptide‐based hybrid nanocapsules are prepared via interfacial polyaddition containing a water‐soluble dye that is efficiently encapsulated inside. The influence of the synthetic parameters as the functionality of the peptide and the nature of the dispersed phase on the structure of the nanocapsules were investigated. After redispersion in water, the enzymatic cleavage of the peptide sequence and the release of the fluorescent dye are both monitored in real time. This is evidenced because of the quenching FRET system framing the recognition site in the peptide sequence, and the fluorescence recovery of the self‐quenched encapsulated dye respectively.