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Fluorescent Core‐Shell Star Polymers Based Bioassays for Ultrasensitive DNA Detection by Surface Plasmon Fluorescence Spectroscopy
Author(s) -
Feng Chuan Liang,
Yin Meizhen,
Zhang Di,
Zhu Shenmin,
Caminade Anne Marie,
Majoral Jean Pierre,
Müllen Klaus
Publication year - 2011
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201000788
Subject(s) - fluorescence , materials science , perylene , dendrimer , fluorescence spectroscopy , spectroscopy , detection limit , surface plasmon , analytical chemistry (journal) , acceptor , förster resonance energy transfer , layer (electronics) , photochemistry , plasmon , chemistry , optoelectronics , nanotechnology , optics , physics , polymer chemistry , condensed matter physics , chromatography , quantum mechanics
Multilayers containing a perylene diimide labelled star polymers (FSP) donor adjacent to phosphorus dendrimer layer on a silver substrate were constructed by layer by layer (LBL) approach. Using Surface Plasmon Enhanced Fluorescence Spectroscopy (SPFS) technique, a time‐resolved ultrasensitive and selective detection of DNA targets relying on enhanced optical fields associated with energy transfer (ET) were achieved under the excitation at 543 nm. The detection limit is about 8 orders of magnitude better than the achieved one under the excitation at 632 nm, which is ascribed to no energy transfer from the donor to the acceptor under the excitation at 632 nm, resulting in much weak detection signal in turn.