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Macromolecular Recognition: Discrimination between Human and Bovine Serum Albumins by Cyclodextrins
Author(s) -
Oi Wataru,
Isobe Mio,
Hashidzume Akihito,
Harada Akira
Publication year - 2011
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201000699
Subject(s) - two dimensional nuclear magnetic resonance spectroscopy , isothermal titration calorimetry , circular dichroism , nuclear overhauser effect , bovine serum albumin , chemistry , human serum albumin , spectroscopy , macromolecule , titration , nuclear magnetic resonance spectroscopy , fluorescence spectroscopy , serum albumin , fluorescence , stereochemistry , crystallography , biochemistry , organic chemistry , physics , quantum mechanics
The interactions of cyclodextrins (CDs) with human serum albumin (HSA) and bovine serum albumin (BSA) were investigated by two‐dimensional nuclear Overhauser effect spectroscopy (2D NOESY), steady state fluorescence, isothermal titration calorimetry, and circular dichroism spectroscopy. 2D NOESY indicates that α‐CD and β‐CD, but not γ‐CD, interact with the aromatic amino acid residues on HSA. On the other hand, the 2D NOESY signals between CDs and BSA are not significantly correlated. Hence, CDs can discriminate the subtle protein structural differences of HSA and BSA.