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Assembly of Anionic Conjugated Polymer with 6‐ O ‐Modified PNP‐ β ‐Galactoside for Fluorescence Logic‐signal‐based Multiplex Detections of Enzymes
Author(s) -
Wang Yajuan,
Xin Baojuan,
Duan Xinrui,
Xing Guowen,
Wang Shu
Publication year - 2010
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201000165
Subject(s) - conjugated system , lipase , multiplex , fluorescence , substrate (aquarium) , galactoside , chemistry , enzyme , polymer , combinatorial chemistry , biochemistry , organic chemistry , biology , optics , physics , bioinformatics , ecology
Anionic conjugated polymer (PFP‐SO 3 − ) was assembled with a novel enzymatic substrate 6‐ O ‐modified PNP‐ β ‐galactoside ( 1 ) for sensitive multiplex enzyme detections. The PFP‐SO 3 − / 1 /lipase/ β ‐galactosidase system has two chemical input signals which are Input 1 (lipase) and Input 2 ( β ‐galactosidase), and output optical signals such as fluorescence emission at 416 nm or 450 nm. Four types of logic gates, including YES, INH, NAND and AND, were successfully constructed and utilized for multiplex detections of lipase and β ‐galactosidase in one tube.