Premium
Efficient DNA Binding and Condensation Using Low Molecular Weight, Low Charge Density Cationic Polymer Amphiphiles
Author(s) -
Mindemark Jonas,
Bowden Tim
Publication year - 2010
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201000141
Subject(s) - cationic polymerization , macromolecule , polymer , charge density , dna condensation , dna , amphiphile , polymer chemistry , condensation , materials science , chemistry , copolymer , combinatorial chemistry , organic chemistry , biochemistry , thermodynamics , physics , transfection , quantum mechanics , gene
A new class of biodegradable cationic macromolecules for DNA binding and condensation was developed by end‐group‐functionalization of poly(trimethylene carbonate). A series of one‐ and two‐armed structures was synthesized and their interaction with DNA was evaluated. To aid data interpretation, a non‐linear modeling method was applied to show efficient DNA binding that was intimately related to cationic charge density and macromolecular architecture. One‐armed, low charge density structures were consistently found to bind to DNA at lower charge ratios than their two‐armed, high charge density counterparts. This suggests that polymer backbone structure and characteristics are important considerations in the development of efficient cationic polymer systems for DNA condensation and delivery.