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Sensing Diffraction Gratings of Antigen‐Responsive Hydrogel for Human Immunoglobulin‐G Detection
Author(s) -
Ye Gang,
Yang Canqiang,
Wang Xiaogong
Publication year - 2010
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201000082
Subject(s) - materials science , diffraction , diffraction grating , antigen , diffraction efficiency , optics , immunoglobulin g , polymer , fluorophore , optoelectronics , grating , fluorescence , immunology , physics , composite material , biology
Sensing diffraction gratings of antigen‐responsive hydrogel were developed for human immunoglobulin‐G (H‐IgG) detection. The IgG‐sensing gratings were fabricated by soft lithographic duplication of photoinduced surface relief gratings on azo polymer films to the hydrogel surfaces. The quantitative detection of H‐IgG was achieved by detecting modulation of the diffraction efficiency when the gratings were exposed to antigen solutions. The gratings showed high sensitivity and specificity for the H‐IgG detection. The detection could be performed at a reasonably fast rate with good reusability. In the process, no fluorophore labeling or additional signal enhancement was required.