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pDNA/poly( L ‐lysine) Polyplexes Functionalized with a pH‐Sensitive Charge‐Conversional Poly(aspartamide) Derivative for Controlled Gene Delivery to Human Umbilical Vein Endothelial Cells
Author(s) -
Sanjoh Mai,
Hiki Shigehiro,
Lee Yan,
Oba Makoto,
Miyata Kanjiro,
Ishii Takehiko,
Kataoka Kazunori
Publication year - 2010
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.201000056
Subject(s) - transfection , umbilical vein , endosome , biophysics , gene delivery , lysine , chemistry , cytotoxicity , cytoplasm , microbiology and biotechnology , biochemistry , in vitro , intracellular , biology , gene , amino acid
An efficient endosome‐escaping function was integrated into the polyplex of plasmid DNA (pDNA) with poly( L ‐lysine) (PLys) to improve its gene transfection efficiency through electrostatic coating with charge‐conversional polymer (CCP). CCP showed charge‐conversional function responding to endosomal pH, leading to the release of pDNA/PLys polyplex into the cytoplasm. The cells took up the intact CCP‐integrated ternary polyplex, which exerted appreciably higher transfection efficiency with lower cytotoxicity than pDNA/PLys polyplex against human umbilical vein endothelial cells (HUVECs). This is consistent with the facilitated endosomal escape of the CCP‐integrated ternary polyplex compared to the pDNA/PLys polyplex as directly observed with confocal laser‐scanning microscopy.