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Synthesis of Guanidinium‐Modified Hyaluronic Acid Hydrogel
Author(s) -
Varghese Oommen P.,
Kisiel Marta,
MartínezSanz Elena,
Ossipov Dmitri A.,
Hilborn Jöns
Publication year - 2010
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200900906
Subject(s) - biopolymer , hydrazide , chemistry , guanidine , hyaluronic acid , reagent , polymer chemistry , polymer , aqueous solution , self healing hydrogels , combinatorial chemistry , organic chemistry , genetics , biology
In this report, a new guanidinylating reagent is presented, which was developed without any protection/deprotection strategy and was successfully employed for linking to hyaluronan in aqueous solution. The dually functionalised HA biopolymer bearing guanidinium and hydrazide groups was synthesised to form hydrogel in less than a minute when mixed with aldehyde‐modified HA. This hydrogel exhibited higher storage modulus with enhanced stability in PBS when compared to the non‐guanidine‐containing gel. The gel shift assay showed that this biopolymer formed a stable complex with DNA as well as efficient gene transfection to cells that express HA‐receptor CD44. The toxicity studies of this polymer with fibroblast cells revealed that the cells were almost 80% viable after 4 d of incubation at high HA concentration (2.5 × 10 −3 M ).