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Novel Triblock Oligopeptides as Efficient Nonviral Vectors: Characterisation and Further Insights
Author(s) -
Seow Wei Yang,
Yang YiYan,
George Andrew J. T.
Publication year - 2010
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200900883
Subject(s) - oligopeptide , transfection , gene delivery , gene transfer , confocal microscopy , biophysics , peptide , gene , confocal , förster resonance energy transfer , transplantation , dna , chemistry , microbiology and biotechnology , biology , computational biology , fluorescence , biochemistry , medicine , physics , optics , surgery
The delivery of immunomodulatory genes into the cornea prior to transplantation is one promising strategy to improve graft survival rates. We recently reported a class of novel triblock oligopeptides that could mediate efficient gene transfer into corneal endothelial cells. Now these peptides are characterised further and it is show that they lack distinct secondary structures. Peptide complexes are also demonstrated to be weakly haemolytic and transfection efficiency is shown to be sensitive to several experimental conditions. SEM and FRET confocal images are used to study the particle morphology and to show that they condense their DNA cargo well.