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Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles
Author(s) -
Miletić Nemanja,
Abetz Volker,
Ebert Katrin,
Loos Katja
Publication year - 2010
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200900497
Subject(s) - candida antarctica , hydrolysis , polystyrene , lipase , chemistry , adsorption , nanoparticle , immobilized enzyme , chromatography , buffer solution , buffer (optical fiber) , nuclear chemistry , enzyme , organic chemistry , polymer , materials science , nanotechnology , telecommunications , computer science
Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal‐B) and on the hydrolytic activity (hydrolysis of p ‐nitrophenyl acetate) of the immobilized Cal‐B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal‐B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435.