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Amplified Fluorescence Turn‐On Assay for Mercury(II) Detection and Quantification based on Conjugated Polymer and Silica Nanoparticles
Author(s) -
Wang Yusong,
Liu Bin
Publication year - 2009
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200800727
Subject(s) - detection limit , mercury (programming language) , thymine , conjugated system , fluorescence , calibration curve , polymer , nanoparticle , chemistry , colloidal gold , cationic polymerization , dna , analytical chemistry (journal) , materials science , chromatography , nanotechnology , polymer chemistry , organic chemistry , optics , biochemistry , physics , computer science , programming language
Abstract An amplified fluorescence turn‐on assay for mercury(II) detection and quantification was developed. This method makes use of specific thymine/mercury(II)/thymine coordination to capture Fl‐labeled DNA onto NP surface. Addition of a cationic conjugated polymer leads to an amplified Fl signal in solution. A sigmoidal Hg 2+ working curve is obtained at fixed [NP] with a detection limit of 0.1 × 10 −6 M . However, by reducing [Hg 2+ ] and [NP] simultaneously, while maintaining [Hg 2+ ]:[DNA duplex] = 3:1, a linear calibration curve is observed with a detection limit of 5 × 10 −9 M . The CCP‐assisted mercury(II) assay shows potential applications in environmental mercury detection and for industrial process control.