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Synthesis of Branched Polyglucans by the Tandem Action of Potato Phosphorylase and Deinococcus geothermalis Glycogen Branching Enzyme
Author(s) -
van der Vlist Jeroen,
Palomo Reixach Marta,
van der Maarel Marc,
Dijkhuizen Lubbert,
Schouten Arend Jan,
Loos Katja
Publication year - 2008
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200800248
Subject(s) - glycogen phosphorylase , branching (polymer chemistry) , glycogen branching enzyme , enzyme , chemistry , amylose , stereochemistry , tandem , glycogen synthase , glycogen , biochemistry , organic chemistry , materials science , composite material , starch
An enzymatic tandem reaction is described in which the enzymes phosphorylase and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) catalyze the synthesis of branched polyglucans from glucose‐1‐phosphate (G‐1‐P). Phosphorylase consumes G‐1‐P and polymerizes linear amylose while Dg GBE introduces branching points on the α ‐(1 → 6) positions by reshuffling short oligosaccharides. The resulting branched polyglucans have an unusually high degree of branching of 11%.