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Cationic and Anionic Conjugated Polyelectrolytes: Aggregation‐Mediated Fluorescence Energy Transfer to Dye‐Labeled DNA
Author(s) -
Jin Youngeup,
Yang Renquiang,
Suh Hongsuk,
Woo Han Young
Publication year - 2008
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200800215
Subject(s) - förster resonance energy transfer , cationic polymerization , chemistry , polyelectrolyte , acceptor , photochemistry , fluorescence , conjugated system , fluorene , polymer , polymer chemistry , organic chemistry , physics , quantum mechanics , condensed matter physics
An electrostatic complex of water‐soluble conjugated polyelectrolytes (CPs) between anionic poly(9,9‐bis(4′‐sulfonatobutyl)fluorene‐ co‐alt ‐1,4‐phenylene) disodium salt (a‐PFP) and cationic poly(9,9‐bis((6′‐ N,N,N ,‐trimethylammonium)hexyl)fluorene‐ co ‐2,1,3‐bezothiadiazole) dibromide (85:15) (c‐PFB 15 ) was tested as a fluorescence resonance energy transfer (FRET) donor to Texas Red (TR)‐labeled single‐stranded DNA (ssDNA‐TR) via two‐step FRET processes. Electrostatic complexation of a‐PFP and c‐PFB 15 in water leads to aggregation of polymer chains, a concomitant reduction of intersegment distances, and energy transfer to the benzothiadiazole (BT) segments. The following complexation with ssDNA‐TR leads to energy transfer from BT to TR via two‐step FRET processes. This detection schematic shows an FRET‐induced signal amplification, which can be achieved by adjusting the charge ratio in the cationic/anionic CP complex and controlling the number density of the binding CPs around the acceptor, resulting in enhanced antenna effects and sensitivity in CP‐based FRET DNA detection assays.