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Porous Protein‐Based Nanoparticle Hydrogel for Protein Chips with Improved Sensitivity
Author(s) -
Tanaka Hiroyuki,
Isojima Tatsushi,
Hanasaki Minako,
Ifuku Yasuo,
Takeuchi Hisao,
Kawaguchi Haruma,
Shiroya Toshifumi
Publication year - 2008
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200800090
Subject(s) - nanoparticle , polymer , colloidal gold , materials science , nanotechnology , substrate (aquarium) , oceanography , geology , composite material
We have discovered a novel method to prepare a protein‐based hydrogel, that is, a ‘three‐dimensional nanostructured protein hydrogel’ (3D NPH), which is composed of loosely inter‐connected protein–polymer hybrid nanoparticles. The 3D NPH can be easily prepared by spotting a protein/polymer mixture on a substrate. Surprisingly, gold nanoparticles carrying protein molecules easily diffuse into the 3D NPH through pores and spaces. We have shown that the protein chip made by our 3D NPH method has tremendously improved sensitivity in detecting protein–protein interactions compared with that by direct protein immobilization methods.