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Process Engineering for Production of Chiral Hydroxycarboxylic Acids from Bacterial Polyhydroxyalkanoates
Author(s) -
Ren Qun,
Ruth Katinka,
ThönyMeyer Linda,
Zinn Manfred
Publication year - 2007
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200700389
Subject(s) - polyhydroxyalkanoates , degradation (telecommunications) , chemostat , centrifugation , bacteria , incubation , chemistry , biodegradation , fermentation , biochemistry , chromatography , biology , organic chemistry , telecommunications , genetics , computer science
An efficient process for the production of ( R )‐hydroxycarboxylic acids (RHAs) from polyhydroxyalkanoates (PHAs) was developed. It involved the synthesis of PHA in bacteria, followed by bringing the culture broth directly to a pH optimal for in vivo PHA degradation, thus avoiding cell collection by centrifugation and pellet resuspension. The optimal pH was maintained to allow maximal release of RHAs. Using this process, cells having a dry weight ( w ) of 1.8 g · L −1 and 45% (w/w) PHA exhibited a linear PHA degradation rate of about 0.059 g · L −1 · h −1 in the first 9 h. Concomitantly, RHAs were released with a rate of 0.057 g · L −1 · h −1 . Further incubation of up to 15 h resulted in almost 90% (w/w) degradation of PHA. Based on this approach in combination with chemostat and a plug flow reactor a continuous process for the production of RHAs could be achieved.