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Piezoelectric Ink‐Jet Printing of Horseradish Peroxidase: Effect of Ink Viscosity Modifiers on Activity
Author(s) -
Di Risio Sabina,
Yan Ning
Publication year - 2007
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200700226
Subject(s) - horseradish peroxidase , viscosity , carboxymethyl cellulose , chemistry , chemical engineering , piezoelectricity , materials science , polymer , pulmonary surfactant , polymer chemistry , composite material , organic chemistry , enzyme , biochemistry , engineering , sodium
In this study a bioink containing the horseradish peroxidase (HRP) enzyme was formulated and jetted with a piezoelectric‐based ink‐jet deposition system. A number of polymers with different molecular weights, functional groups and electric charge were tested as viscosity modifiers. The impact of viscosity modifiers on HRP activity was systematically studied. It was found that viscosity modifiers typically used in commercial ink formulations significantly impaired HRP activity possibly due to limitations in diffusion. In contrast, carboxymethyl cellulose increased viscosity to the desired range for piezoelectric jetting without negatively affecting the HRP activity. Good jettability was obtained for the HRP bioink by adding a suitable surfactant, a viscosity modifier and a humectant.