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Sialic Acid Engineering of Thin Hydrogel Membranes
Author(s) -
Woerly Stéphane,
Fort Sébastien,
Heyraud Alain
Publication year - 2007
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200700024
Subject(s) - methacrylamide , self healing hydrogels , methacrylate , acrylate , membrane , sialic acid , polymer chemistry , chemistry , radical polymerization , phenylboronic acid , copolymer , polymer , biochemistry , organic chemistry , acrylamide , catalysis
We report the synthesis of glycosylated hydrogel membranes of poly{[ N ‐(2‐hydroxypropyl)methacrylamide]‐ co ‐(2‐hydroxyethyl methacrylate)} with the aim of developing bioactivated polymer substrates for cell culture. 3′‐Sialyllactose, the saccharidic portion of the GM 3 ganglioside involved in cell‐cell recognition over a wide range of biological processes, was chemically modified with an acrylate group and incorporated into the growing macromolecular network of hydrogels by free radical crosslinking copolymerization. The incorporation and accessibility of the sialic acid residues at the hydrogel surface was verified by enzyme linked immunosorbent assay using mouse monoclonal anti‐GM 3 , and by electron microscopy after labeling with lectin‐gold nanoparticles. The water content was further characterized by thermogravimetry.