Premium
Characterization of the Mode of Incorporation of Lipophilic Compounds in Solid Dispersions at the Nanoscale Using Fluorescence Resonance Energy Transfer (FRET)
Author(s) -
van Drooge Dirk Jan,
Braeckmans Kevin,
Hinrichs Wouter L. J.,
Remaut Katrien,
De Smedt Stefaan C.,
Frijlink Henderik W.
Publication year - 2006
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200600177
Subject(s) - förster resonance energy transfer , bodipy , acceptor , fluorescence , materials science , polyvinylpyrrolidone , absorption (acoustics) , photochemistry , nanoscopic scale , absorption spectroscopy , resonance (particle physics) , analytical chemistry (journal) , chemistry , nanotechnology , organic chemistry , polymer chemistry , optics , physics , particle physics , condensed matter physics , composite material
Summary: Efficient engineering of solid dispersions stagnates by the current inability to establish the mode of drug distribution on a molecular level at a low drug load. This study describes the application of fluorescence resonance energy transfer (FRET) to characterize the mode of incorporation of dispersed lipophilic molecules in a solid matrix. Two different lipophilic fluorophores (donor and acceptor) were used as model substances and were incorporated in polyvinylpyrrolidone to form solid dispersions using two different production processes: lyophilization and fusion. The efficiency of the resonance energy transfer from donor to acceptor was measured by confocal microscopy. We show that the method can be used to compare the modes of drug incorporation of solid dispersions at the nanoscale.Absorption and emission spectra of Bodipy R6G (donor) and Bodipy 650/665 (acceptor).