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Patterning Proteins on Surfaces of Cross‐Sectioned Multilayer Polymer Films
Author(s) -
Zhang Chun,
Husson Scott M.,
Hirt Douglas E.
Publication year - 2006
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200600144
Subject(s) - polymer , materials science , copolymer , acrylic acid , streptavidin , fluorescence , template , polyethylene , micrograph , polymer chemistry , polymer science , nanotechnology , chemical engineering , composite material , scanning electron microscope , optics , biotin , chemistry , biochemistry , engineering , physics
Summary: A new approach is introduced to create submicrometer patterned surfaces using multilayer polymer films that contain alternating layers of two polymers, linear low‐density polyethylene (LLDPE) and ethylene‐ co ‐(acrylic acid) copolymer (EAA). Patterned templates have been prepared by microtoming the multilayer molded sheets. Regionally confined chemical functionality is confirmed by grafting an amine‐terminated biotin and adsorbing streptavidin specifically on the alternating layers of EAA.Fluorescence micrograph of the Alexa488‐streptadivin patterned polymer surface.