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Red Blood Cell Templated Polyelectrolyte Capsules: A Novel Vehicle for the Stable Encapsulation of DNA and Proteins
Author(s) -
Kreft Oliver,
Georgieva Radostina,
Bäumler Hans,
Steup Martin,
MüllerRöber Bernd,
Sukhorukov Gleb B.,
Möhwald Helmuth
Publication year - 2006
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200500777
Subject(s) - polyelectrolyte , confocal , confocal microscopy , fluorescence , nucleic acid , fluorescence microscope , fluorescence spectroscopy , human serum albumin , biophysics , cell encapsulation , dna , fluorescence correlation spectroscopy , chemistry , fluorescence lifetime imaging microscopy , confocal laser scanning microscopy , microscopy , chromatography , polymer , self healing hydrogels , biochemistry , polymer chemistry , biology , organic chemistry , optics , physics , microbiology and biotechnology
Summary: A novel method for the encapsulation of biomacromolecules, such as nucleic acids and proteins, into polyelectrolyte microcapsules is described. Fluorescence‐labelled double‐stranded DNA and human serum albumin (HSA) are used as model substances for encapsulation in hollow microcapsules templated on human erythrocytes. The encapsulation procedure involves an intermediate drying step. The accumulation of DNA and HSA in the capsules is observed by confocal laser scanning microscopy, UV spectroscopy, and fluorimetry. The mechanism of encapsulation is discussed.Confocal fluorescence microscopy images of encapsulated TRITC‐HSA (left) and dsDNA (right). Inserts demonstrate fluorescence profiles for both compounds.