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Construction of Polycation‐Based Non‐Viral DNA Nanoparticles and Polyanion Multilayers via Layer‐by‐Layer Self‐Assembly
Author(s) -
Ren Kefeng,
Ji Jian,
Shen Jiacong
Publication year - 2005
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200500482
Subject(s) - nanoparticle , ethyleneimine , dna , materials science , layer by layer , layer (electronics) , polyelectrolyte , nanotechnology , self assembly , polymer , chemical engineering , chemistry , biochemistry , composite material , engineering
Summary: The multilayers of polycation‐based non‐viral DNA nanoparticles and biodegradable poly( L ‐glutamic acid) (PGA) were constructed by a layer‐by‐layer (LbL) technique. Poly(ethyleneimine) (PEI) was used to condense DNA to develop non‐viral DNA nanoparticles. AFM, UV‐visible spectrometry, and TEM measurements revealed that the PEI‐DNA nanoparticles were successfully incorporated into the multilayers. The well‐structured, easily processed multilayers with the non‐viral DNA nanoparticles may provide a novel approach to precisely control the delivery of DNA, which may have great potential for gene therapy applications in tissue engineering, medical implants, etc.A TEM image of the cross section of a (PGA/PEI‐DNA nanoparticle) 20 multilayer.