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Polyelectrolyte Multilayer Microspheres as Carriers for Bienzyme System: Preparation and Characterization
Author(s) -
Balabushevich Nadezda G.,
Sukhorukov Gleb B.,
Larionova Natalia I.
Publication year - 2005
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200500141
Subject(s) - polyelectrolyte , dextran , glucose oxidase , adsorption , chemical engineering , microsphere , materials science , immobilized enzyme , chromatography , chemistry , polymer chemistry , biosensor , nanotechnology , polymer , organic chemistry , enzyme , engineering
Summary: Polyelectrolyte multilayer microspheres were prepared by alternating adsorption of dextran sulfate and protamine on melamine formaldehyde cores followed by the partial decomposition of the core. Peroxidase and glucose oxidase were immobilized in the prepared microspheres. Retention of enzymatic activity of the peroxidase/glucose oxidase system incorporated into the microspheres was demonstrated. Applicability of the bienzyme system immobilized in the microspheres for kinetic glucose assay was shown.SEM image of the polyelectrolyte multilayer microsphere.