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Linear Combinatorial Synthesis with Fourier Transform Library Analysis
Author(s) -
Schwabacher Alan W.,
Johnson Christopher W.,
Geissinger Peter
Publication year - 2004
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/marc.200300157
Subject(s) - schematic , computer science , fourier transform , thread (computing) , algorithm , mathematics , electronic engineering , engineering , mathematical analysis , operating system
Feature Article: Combinatorial synthesis procedures that fit a restrictively defined “fully parallel” criterion tend to be extremely efficient methods. We describe how linear library organization allows such synthesis, without loss of synthetic history information, with an example of a peptide library. Fluorescence measurements of several types are used to measure activities. A novel Fourier Transform approach to library data analysis allows robust evaluation of trends. The use of the cladding of optical fibers as linear supports for combinatorial libraries significantly extends the potential applications of the technique, allowing for spatially resolved optical evaluation of library activity using laser pulses propagating through the fiber core. Moreover, by using different fiber cladding materials, the range of chemistries amenable to one‐dimensional combinatorial synthesis is significantly increased. It is noteworthy that the procedure is particularly applicable to the fabrication and evaluation of real‐time sensor arrays.Schematic representation of a fully parallel synthesis on a linear solid support. The result is a sequence of regions on the thread, each bearing a library member.