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Effect of Poly(trehalose methacrylate) Molecular Weight and Concentration on the Stability and Viscosity of Insulin
Author(s) -
Gelb Madeline B.,
Maynard Heather D.
Publication year - 2021
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.202100197
Subject(s) - trehalose , insulin , viscosity , polymer , methacrylate , materials science , intrinsic viscosity , mole , polymer chemistry , nuclear chemistry , chromatography , chemistry , biochemistry , organic chemistry , biology , endocrinology , copolymer , composite material
Instability to storage and shipping conditions and injection administration remain major challenges in treating chronic conditions with biopharmaceuticals. Herein, formulations of poly(trehalose methacrylate) (pTrMA) are successfully optimized to stabilize insulin without appreciably increasing viscosity. Polymers are synthesized (2400–29 200 Da), and added to insulin at different concentrations. pTrMA maintained >95% intact insulin against 250 rpm at 37 °C for 3 h with at least 10 mol. equiv. of 5.0 kDa, 7.5 mol. equiv. of 9.4 kDa, 5 mol. equiv. of 12.8 kDa, 1 mol. equiv. of 19.8 kDa, and 0.5 mol. equiv. of 29.2 kDa polymers, compared to 13.1% of insulin alone. The lowest pTrMA concentration formulations are more viscous than insulin alone, but the highest viscosity, U‐600 with 10 mol. equiv. of 5 kDa pTrMA, is only 1.43 cP at 25 °C. This data demonstrates that pTrMA is a promising low viscosity additive to stabilize the diabetes therapeutic insulin.