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Design of a Bio‐Based Device for Micro Total Analysis Combining Fused Deposition Modeling and Layer‐by‐Layer Technologies
Author(s) -
León Alberto S.,
Frutos Raquel M.,
Molina Sergio I.
Publication year - 2020
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.202000461
Subject(s) - layer by layer , materials science , layer (electronics) , chitosan , biomolecule , nanotechnology , deposition (geology) , polymer , adsorption , polylactic acid , sodium alginate , chemical engineering , organic chemistry , chemistry , sodium , composite material , engineering , paleontology , sediment , metallurgy , biology
In this paper a methodology is presented to fabricate a micropatterned device made of bio‐based polymers (polylactic acid, sodium alginate (ALG), and chitosan (CHI)) suitable for micro total analysis. Fused deposition modeling and layer‐by‐layer technologies are combined to create tailorable devices with submillimetric controlled well size that can be rapidly prepared in house. As proof of concept, the influence of immobilization of ALG/CHI bilayers in the selective adsorption of alkaline phosphatase and its activity is studied. Due to the noncovalent nature of these interactions, it is proven that these devices can be reused for at least three times, contributing to generate less waste. It is foreseen that the combination of these fast and inexpensive technologies will lead to new setups for laboratory and clinical assays such as high‐throughput screening where controlled immobilization of enzymes, peptides, or other valuable biomolecules needs to be used.