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Synthesis of Strong Cation Exchange Macroporous Polymer Cluster for Convective Protein Chromatography
Author(s) -
Lorenz Marcel,
Vogg Sebastian,
Finkelstein Patrick,
Storti Giuseppe,
Morbidelli Massimo
Publication year - 2019
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.201900311
Subject(s) - polymerization , chain transfer , methacrylate , polymer , materials science , chromatography , mass transfer , cycloaddition , convective flow , cationic polymerization , chemical engineering , polymer chemistry , chemistry , convection , catalysis , radical polymerization , organic chemistry , physics , engineering , composite material , thermodynamics
Alkyne‐terminated polyelectrolytic brushes based on 3‐sulfopropyl methacrylate are synthesized by reversible addition‐fragmentation chain transfer polymerization and subsequently linked to macroporous polymeric microclusters by Cu(I)‐catalyzed azide alkyne cycloaddition. It is shown that a column packed with the obtained material enables convective cation‐exchange chromatography of proteins, exhibiting flow rate independent resolution and dynamic binding capacity (8 mg mL −1 ). These flow characteristics are similar to those of monoliths and membranes and therefore improve the mass transport of existing chromatography resins used in the downstream process of therapeutic proteins, while the positive aspects of chromatographic beads are maintained, facilitating implementation in well‐developed platform technologies.