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Effects of Silk Degumming Process on Physicochemical, Tensile, and Optical Properties of Regenerated Silk Fibroin
Author(s) -
Nultsch Kira,
Bast Livia K.,
Näf Muriel,
Yakhlifi Salima El,
Bruns Nico,
Germershaus Oliver
Publication year - 2018
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.201800408
Subject(s) - fibroin , silk , materials science , ultimate tensile strength , sericin , sodium carbonate , sodium , vulcanization , chemical engineering , yield (engineering) , composite material , natural rubber , metallurgy , engineering
Sericin removal from silk (degumming) affects material characteristics of silk fibroin (SF). Sodium carbonate is most commonly used for degumming, but numerous alternative methods are available. Herein, a systematic comparison of degumming methods is provided. Sodium carbonate, sodium oleate, trypsin, and ionic liquid are used, and materials are characterized regarding mass loss, SF content, molecular integrity of SF, refractive index, and tensile properties. Complete degumming is achieved within 30 min of using sodium carbonate, but results in significant reduction of molecular weight, shift toward less acidic charge variants, and reduction of yield‐ and rupture force. Sodium oleate and trypsin are inefficient and negatively affect tensile properties, while ionic liquid shows good efficiency and marginal degradation of SF but also reduced yield‐ and rupture force. Refractive index is not affected by degumming. These results allow rational selection of the degumming method and tuning of SF properties for biomedical applications.