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Incorporation of Polycaprolactone to Cyclodextrin‐Based Nanocarrier for Potent Gene Delivery
Author(s) -
Fan Xiaoshan,
Cheng Hongwei,
Wu Yihong,
Loh Xian Jun,
Wu YunLong,
Li Zibiao
Publication year - 2018
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.201800255
Subject(s) - transfection , gene delivery , polyethylenimine , polycaprolactone , nanocarriers , materials science , cyclodextrin , cationic polymerization , amphiphile , methacrylate , biophysics , lipofectamine , micelle , copolymer , chemistry , drug delivery , polymer chemistry , nanotechnology , biochemistry , organic chemistry , polymer , gene , biology , vector (molecular biology) , aqueous solution , composite material , recombinant dna
Stability of polyplex and safety are key factors to achieve stable gene transfection and high transfection efficiency. In this report, a star‐like amphiphilic biocompatible cyclodextrin‐poly(ε‐caprolactone)‐poly(2‐(dimethylamino) ethyl methacrylate), β‐CD‐ g ‐(PCL‐ b ‐PDMAEMA) x copolymer, consisting of biocompatible cyclodextrin core, biodegradable and stable poly(ε‐caprolactone) PCL segments, cationic and hydrophilic PDMAEMA blocks, is synthesized to achieve high efficiency of gene transfection with enhanced stability, due to the micelle formation by hydrophobic PCL segments. In comparison with polyethylenimine (PEI‐25k), a golden standard for nonviral vector gene delivery, this copolymer shows higher encapsulated plasmid desoxyribose nucleic acid (pDNA) ability and the persistence of transgene expression. More interestingly, this gene delivery platform by β‐CD‐ g ‐(PCL‐ b ‐PDMAEMA) x shows lower toxicity but better gene transfection efficiency at low N/P ratios, indicating high potential in gene therapy applications.