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Pore Size Distribution and Blend Composition Affect In Vitro Prevascularized Bone Matrix Formation on Poly(Vinyl Alcohol)/Gelatin Sponges
Author(s) -
De la Ossa Jose Gustavo,
Trombi Luisa,
D'Alessandro Delfo,
Coltelli Maria Beatrice,
Serino Lorenzo Pio,
Pini Roberto,
Lazzeri Andrea,
Petrini Mario,
Danti Serena
Publication year - 2017
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.201700300
Subject(s) - vinyl alcohol , gelatin , materials science , tissue engineering , emulsion , chemical engineering , stromal cell , matrix (chemical analysis) , biomedical engineering , umbilical vein , polymer chemistry , in vitro , composite material , polymer , chemistry , biochemistry , biology , engineering , cancer research , medicine
This study aims at identifying compositional and architectural (pore size and distribution) parameters of biocompatible scaffolds, which can be best suitable for both osteoblasts and endothelial cells to produce optimized 3D cocultured constructs. Spongy scaffolds are prepared using poly(vinyl alcohol) (PVA) and gelatin (G) at different weight compositions (PVA/G range: 100/0–50/50, w/w) via emulsion and freeze‐drying. The higher the gelatin content, the larger is the volume occupied by higher size pores. Human umbilical vein endothelial cells and human mesenchymal stromal cells are independently differentiated on the scaffolds to select the best candidate for the coculture. The results of metabolic activity and histology on single platforms show both cell‐ and material‐type dependent outcomes. PVA/G 80/20 scaffolds are finally selected and allow the formation of mineralized matrix containing organized endothelial‐like structures. This study highlights the need for systematic investigations on multifactorial parameters of scaffolds to improve vascularized bone substitutes.