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Microfluidic Fabrication of Physically Assembled Nanogels and Micrometric Fibers by Using a Hyaluronic Acid Derivative
Author(s) -
Agnello Stefano,
Bongiovì Flavia,
Fiorica Calogero,
Pitarresi Giovanna,
Palumbo Fabio Salvatore,
Di Bella Maria Antonietta,
Giammona Gaetano
Publication year - 2017
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.201700265
Subject(s) - nanogel , materials science , microfiber , fabrication , ethylenediamine , hyaluronic acid , surface modification , ionic strength , microfluidics , chemical engineering , polymer chemistry , nanotechnology , aqueous solution , drug delivery , composite material , chemistry , organic chemistry , medicine , alternative medicine , pathology , biology , engineering , genetics
The employ of a hyaluronic acid (HA) derivative, bearing octadecyl (C 18 ) and ethylenediamine (EDA) groups, for microfluidic fabrication of nanogels and microfibers is reported in this study. Two HA‐EDA‐C 18 derivatives (125 and 320 kDa) having ionic strength sensitive properties are synthesized and characterized. The control of the rheological properties of HA‐EDA‐C 18 aqueous dispersions by formation of inclusion complexes with hydroxypropyl‐β‐cyclodextrins (HPCD) is described. Reversibility of C 18 /HPCD complexation and physical crosslinking is detected in media with different ionic strength through oscillation frequency tests. HA‐EDA‐C 18 125 kDa is employed for nanogel fabrication. Control over nanogel dimension by flow ratio regulation is demonstrated. HA‐EDA‐C 18 320 kDa with HPCD is employed for fabrication of both microfibers and microchannels. Dimension of fibers is controlled by modulating flow ratios. Suitability for biological functionalization is assayed introducing cell adhesive peptides. Adhesion and encapsulation of human umbilical vein endothelial cells is evaluated.