Premium
In Situ Crosslinking of Highly Porous Chitosan Scaffolds for Bone Regeneration: Production Parameters and In Vitro Characterization
Author(s) -
Kruppke Benjamin,
Farack Jana,
Sommer Freya,
Weil Simy,
Aflalo Eliahu David,
Wiesmann HansPeter,
Sagi Amir,
Hanke Thomas
Publication year - 2017
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.201700147
Subject(s) - chitosan , materials science , glutaraldehyde , simulated body fluid , scaffold , chemical engineering , porosity , scanning electron microscope , context (archaeology) , in situ , brushite , biomedical engineering , calcium , composite material , chemistry , chromatography , organic chemistry , medicine , paleontology , engineering , metallurgy , biology
Various methods of chitosan scaffold production are reported in the literature so far. Here, in situ crosslinking with glutaraldehyde is reported for the first time. It combines pore formation and chitosan crosslinking in a single step. This combination allows incorporation of fragile molecules into 3D porous chitosan scaffolds produced by simple and gentle lyophilization. In this study, parameters of in situ crosslinking of porous chitosan scaffold formation as well as their effect on degradation and bioactivity of the scaffolds are examined. The scaffolds are characterized in the context of their prospective application as bone substitute material. The addition of calcium phosphate phases (hydroxyapatite, brushite) to the macroporous chitosan scaffolds allows manipulation of the bioactivity that is investigated by incubation in simulated body fluid (SBF). The bioactivity is significantly influenced by the modus of changing the fluid (static, daily‐, and twice‐a‐week change). Scaffolds are morphologically characterized by means of scanning electron microscopy, and the mechanical stability is tested after incubation in SBF and phosphate‐buffered saline.