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Flow‐ T hrough‐ M ediated Surface Immobilization of Sub‐ M icrometre Particles in Monolithic Cryogels
Author(s) -
Hajizadeh Solmaz,
Mattiasson Bo,
Kirsebom Harald
Publication year - 2014
Publication title -
macromolecular materials and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.913
H-Index - 96
eISSN - 1439-2054
pISSN - 1438-7492
DOI - 10.1002/mame.201300358
Subject(s) - adsorption , composite number , materials science , covalent bond , molecule , chemical engineering , composite material , nanotechnology , chemistry , organic chemistry , engineering
Particles with functional groups on their surface were immobilized on premade cryogels through covalent bonding to maximize the accessibility of the target molecules to the surface of the particles. The physical properties of the composite cryogel were characterized by SEM, texture analysis and nitrogen adsorption. Different key factors affecting immobilization were also investigated, e.g., recirculation time and the number of functional groups on the premade cryogels. It was found that the physical characteristics of the composite cryogels prepared in this way were very similar to those of the primary matrices, while their binding capacity increased in comparison with conventionally prepared composite cryogels. The binding capacities of two different types of composite cryogel were evaluated by studying the adsorption of small molecules (copper ions and Orange G) and a large molecule (thermostable His‐tagged exoglucanase).