Premium
Delivering Proapoptotic Peptide by HSP Nanocage for Cancer Therapy
Author(s) -
Wang Dongmei,
Chen Li,
Wang Mingyue,
Cui Meiying,
Huang Lili,
Xia Wei,
Guan Xingang
Publication year - 2020
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.202000003
Subject(s) - nanocages , nanocarriers , peptide , chemistry , heat shock protein , cytotoxicity , nanoparticle , drug delivery , melanoma , geldanamycin , cancer research , nanotechnology , biochemistry , hsp90 , materials science , in vitro , biology , gene , catalysis
Protein nanoparticles have attracted widespread attention in the area of biocatalysis, drug delivery, and vaccine preparation during the past decade. Among various protein nanostructures, protein nanocages have proved to be a multifunctional platform for tumor diagnosis and drug delivery due to easy interior encapsulation and outer surface display. In this report, a protein‐only nanoparticle (HSP‐KLAK) is developed based on small heat shock protein (HSP) nanocage and proapoptotic peptide (KLAK) for enhanced antitumor activity. The HSP‐KLAK nanoparticles show efficient cellular uptake and good lysosomal escape in melanoma cells. Moreover, HSP‐KLAK nanoparticles show significantly enhanced cytotoxicity toward B16F10 melanoma cells compared with free proapoptotic peptide. This study demonstrates that protein nanocage represents an efficient nanocarrier for delivering anticancer peptide into tumors.