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High‐Performance Sieving Electrophoresis for Single‐Nucleotide Polymorphisms with a Structuring Hydrogel Network
Author(s) -
You Qingxiang,
Wang Ping,
Zhang Dawei,
Li Zhenqing,
Yamaguchi Yoshinori
Publication year - 2020
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201900385
Subject(s) - capillary electrophoresis , ethylene glycol , polyacrylamide , peg ratio , chemistry , gel electrophoresis , polymer , electrophoresis , chromatography , tetra , self healing hydrogels , dna , polymer chemistry , biochemistry , organic chemistry , finance , economics , medicinal chemistry
Although DNAs with single‐nucleotide difference (e.g., 1000 and 1001 nt) can be resolved by capillary electrophoresis (CE), separation of the same length DNA with single‐nucleotide difference is still a great challenge, which is caused by the structural or thermodynamical fluctuation of the sieving matrix. Here, a homogeneous tetra‐arm poly (ethylene glycol) (Tetra‐PEG) polymer network is synthesized in a capillary, and single monoprotic ssDNA is successfully separated in the capillary filled with cross‐linked Tetra‐PEG polymer. Results show that the separation performance in both slab‐gel electrophoresis and CE is dramatically improved by Tetra‐PEG gel than polyacrylamide gel as sieving matrix. Moreover, separation based on Tetra‐PEG gel depends on not only the DNA size but also the chemical modification and DNA sequence, indicating that it is an ideal alternative to polyacrylamide gel.