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Metal‐Coupled Fluorescence Resonance Energy Transfer in Layer‐by‐Layer Assemblies for Dual Modality Fluorescence Enhancement
Author(s) -
Kim KiSe,
Yoo Seong Il,
Sohn ByeongHyeok
Publication year - 2018
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201800115
Subject(s) - förster resonance energy transfer , fluorescence , fluorescence in the life sciences , acceptor , chemistry , photochemistry , surface plasmon resonance , resonant inductive coupling , nanotechnology , nanoparticle , materials science , energy transfer , chemical physics , optics , physics , condensed matter physics
Fluorescence resonance energy transfer (FRET) has attracted a great deal of attention in chemical and biological analysis because fluorescence intensity of given fluorophores (acceptor) can be amplified by placing energy‐harvesting molecules (donor) in their vicinity. However, FRET‐based fluorescence has a certain limitation in terms of signal amplification because it inherently relies on the intrinsic properties of donors and acceptors as well as the donor‐to‐acceptor distance. To overcome this limitation, metal nanoparticles (NPs) are introduced to a FRET system to engineer the dipole–dipole interaction in FRET using the localized surface plasmon resonance (LSPR) of metal NPs. A 63.1‐fold fluorescence enhancement is observed using an LSPR‐coupled FRET process based on layer‐by‐layer (LbL) assemblies composed of a pair of donor–acceptor fluorescent dyes and metal NPs. It is discovered that by a) optimizing LSPR‐coupled excitation enhancement in donors as well as emission enhancement in acceptors and b) opening FRET channels between LSPR‐coupled donors and acceptors, dual enhancement mechanisms based on FRET and LSPR can be coupled to induce strong fluorescence. Interestingly, it is noticed that the fluorescence enhancement is achieved with reduced FRET efficiency, which is explained by competitive near‐field interactions in the LSPR‐coupled FRET system.