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Dual‐Stimuli Sensitive Hybrid Materials: Ferritin–PDMAEMA by Grafting‐From Polymerization
Author(s) -
Tebaldi Marli Luiza,
Charan Himanshu,
Mavliutova Liliia,
Böker Alexander,
Glebe Ulrich
Publication year - 2017
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201600529
Subject(s) - lower critical solution temperature , atom transfer radical polymerization , chemistry , methacrylate , polymer , polymer chemistry , polymerization , dynamic light scattering , matrix assisted laser desorption/ionization , ionic strength , sodium dodecyl sulfate , copolymer , desorption , materials science , organic chemistry , nanoparticle , nanotechnology , adsorption , aqueous solution
The combination of stimuli‐responsive polymers and proteins that can transport drugs is a promising approach for drug delivery. The formation of ferritin–poly(2‐dimethylaminoethyl methacrylate) (PDMAEMA) conjugates by atom‐transfer radical polymerization from the protein macroinitiator is described. PDMAEMA is a dual‐stimuli‐responsive polymer and the thermo‐ and pH‐responsive properties of the resulting conjugates are studied in detail with dynamic light scattering (DLS). Additionally, it is demonstrated that the lower critical solution temperature (LCST) of the protein–polymer conjugates can be further adjusted by the ionic strength of the solution. The conjugates are also characterized by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), matrix‐assisted laser desorption ionization‐time of flight (MALDI‐ToF) mass spectrometry, and NMR spectroscopy. The obtained MALDI‐ToF mass spectra are exceptional for protein–polymer conjugates and have not been so often reported.