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Synthetic Mimics of Antimicrobial Peptides (SMAMPs) in Layer‐by‐Layer Architectures: Possibilities and Limitations
Author(s) -
Dorner Franziska,
MalekLuz Alicia,
Saar Julia S.,
Bonaus Sebastian,
AlAhmad Ali,
Lienkamp Karen
Publication year - 2016
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201600268
Subject(s) - polyelectrolyte , layer by layer , zeta potential , cationic polymerization , polymer , surface charge , chemistry , surface plasmon resonance , layer (electronics) , antimicrobial , chemical engineering , acrylic acid , nanotechnology , combinatorial chemistry , materials science , polymer chemistry , organic chemistry , nanoparticle , copolymer , engineering
Polymer‐based synthetic mimics of antimicrobial peptides (SMAMPs) show promising antimicrobial activity in solution and as surface‐attached networks. In this paper, their potential as active ingredients in layer‐by‐layer (LbL) assemblies is evaluated. These consist of the weak, anionic polyelectrolyte poly(acrylic acid), and either the hydrophobic butyl SMAMP or the hydrophilic diamine SMAMP (both of which are cationic, weak polyelectrolytes). In situ surface plasmon resonance spectroscopy is used to optimize the LbL assembly conditions. An “overshooting” is observed when depositing the SMAMP layer. Zeta potential measurements show that the layer charge inversion is reduced at each build‐up step due to layer interpenetration. Thus, the positive charge of LbL assemblies with SMAMPs as the top layer is low; a significant part is consumed to maintain layer stability. This leads to reduced antimicrobial activity. Fine‐tuning of the assembly and post‐treatment conditions leads to SMAMP‐PAA LbL systems with optimized antimicrobial activity and stability.