Premium
Development of a Redox‐Responsive Polymeric Profluorescent Probe
Author(s) -
Hansen KaiAnders,
FairfullSmith Kathryn E.,
Bottle Steven E.,
Blinco James P.
Publication year - 2016
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201600147
Subject(s) - nitroxide mediated radical polymerization , fluorophore , moiety , chemistry , fluorescence , redox , photochemistry , electron paramagnetic resonance , polymer , polymer chemistry , radical polymerization , polymerization , organic chemistry , nuclear magnetic resonance , physics , quantum mechanics
Profluorescent nitroxides (PFNs) have emerged as an important class of imaging agents for monitoring intracellular redox status and levels of oxidative stress. However, the fast reduction of nitroxides upon incubation within cells limits the window of opportunity for detection. By increasing the concentration of nitroxides per fluorophore, their reduction to the corresponding hydroxylamines and the subsequent switch‐on of fluorescence can be delayed. Herein the preparation of nitroxide‐containing polymers of different chain length coupled to a fluorophore is reported and their reduction with pentafluorophenylhydrazine is examined. The fluorescence switch‐on kinetics and radical concentrations are monitored by fluorescence and electron paramagnetic resonance spectroscopy and compared to a conventional PFN bearing a single nitroxide moiety. The polymeric PFNs display significant delays in reduction and fluorescence switch‐on and higher turn‐on ratios than their single‐nitroxide counterparts. The results of this study indicate that polymeric PFNs are a promising architecture for future imaging agents.