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Preparation of pH‐Responsive Amphoteric Glycogen Hydrogels by α‐Glucan Phosphorylase‐Catalyzed Successive Enzymatic Reactions
Author(s) -
Takata Yusei,
Yamamoto Kazuya,
Kadokawa Junichi
Publication year - 2015
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201500151
Subject(s) - chemistry , self healing hydrogels , glycogen phosphorylase , glucan , depolymerization , monomer , polymerization , polymer chemistry , catalysis , glycogen , enzyme , organic chemistry , enzyme catalysis , biochemistry , polymer
The thermostable α‐glucan phosphorylase‐catalyzed enzymatic α‐glucuronylation and subsequent α‐glucosaminylation of glycogen using α‐ d ‐glucuronic acid 1‐phosphate (GlcA‐1‐P) and α‐ d ‐glucosamine 1‐phosphate (GlcN‐1‐P), respectively, produce amphoteric glycogens. The products exhibit the inherent isoelectric points, which change depending on the GlcA/GlcN ratios. Chain elongation of amyloses from the nonfunctionalized, nonreducing ends of the products through the thermostable α‐glucan phosphorylase‐catalyzed enzymatic polymerization of α‐ d ‐glucose 1‐phosphate monomers is demonstrated to yield amphoteric glycogen hydrogels. The produced hydrogels exhibit pH‐responsive behavior.