Premium
Fluorescence Reporting of Binding Interactions of Target Molecules with Core–Shell‐Type Cortisol‐Imprinted Polymer Particles Using Environmentally Responsible Fluorescent‐Labeled Cortisol
Author(s) -
Murase Nobuo,
Taniguchi ShinIchi,
Takano Eri,
Kitayama Yukiya,
Takeuchi Toshifumi
Publication year - 2015
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201500065
Subject(s) - itaconic acid , molecular imprinting , fluorescence , molecularly imprinted polymer , imprinting (psychology) , chemistry , polymer , monomer , polymer chemistry , molecule , biophysics , organic chemistry , biochemistry , selectivity , catalysis , physics , quantum mechanics , biology , gene
Core–shell‐type molecularly imprinted polymer particles (MIP‐NPs) bearing specific binding cavities for cortisol in their shell layers by two‐step emulsifier‐free emulsion polymerizations are synthesized, where both the strategies of semicovalent molecular imprinting and noncovalent molecular imprinting are concurrently performed using methacryloyl cortisol and itaconic acid as a template molecule and a functional monomer, respectively. Newly designed fluorescent reporter molecules, dansyl‐labeled cortisol, to investigate the binding capability of MIP‐NPs toward cortisol by fluorescence measurements are developed. The binding rate constant ( k a ) and affinity constant ( K a ) of MIP‐NPs toward cortisol are successfully estimated by curve fitting for fluorescence spectral shift. The k a value of MIP‐NPs is 1.8 times larger than that of reference MIP‐NPs prepared using only methacryloyl cortisol without itaconic acid (semicovalent imprinting), indicating that the concurrent demonstration of both the semicovalent imprinting and noncovalent imprinting processes is effective for constructing high affinity binding cavities for cortisol.