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Bioactive Chitosan Nanoparticles Loaded with Retinyl Palmitate: A Simple Route Using Ionotropic Gelation
Author(s) -
FernándezGutiérrez Mar,
Bossio Ornella,
GómezMascaraque Laura G.,
VázquezLasa Blanca,
Román Julio San
Publication year - 2015
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201500034
Subject(s) - retinyl palmitate , zeta potential , chitosan , dpph , chemistry , ionotropic effect , antioxidant , nanoparticle , cytotoxicity , chemical engineering , nuclear chemistry , nanotechnology , organic chemistry , materials science , in vitro , biochemistry , retinol , nmda receptor , receptor , vitamin , engineering
Nowadays, there is an increasing demand in incorporating antioxidants into biopolymeric nanoparticles (NPs) in order to enlarge their applications in the pharmaceutical and biomedical fields. The challenge of the work is to investigate the feasibility of encapsulating retinyl palmitate (RP) into chitosan (Ch) NP via ionotropic gelation. Two routes are addressed in the preparation of the systems, and they have an influence on the physicochemical properties of the NP in addition to the antioxidant amount loaded. The resulting RP‐encapsulated NP has pseudo spherical morphology, average hydrodynamic diameters between 230 and 350 nm and zeta potential values between +12.5 and +30 mV, depending on RP load and procedure. Encapsulation efficiency ranges from about 35% to 64%, and the loading capacity between 4% and 10%. The antioxidant activity studied by the 1,1‐diphenyl‐2‐picryl‐hydrazyl (DPPH) assay and cytotoxicity of RP‐loaded NP evaluated against human dermal fibroblasts is dependent on RP content and NP concentration.