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Clickable Poly(ethylene glycol)‐Microsphere‐Based Cell Scaffolds
Author(s) -
Nguyen Peter K.,
Snyder Christopher G.,
Shields Jason D.,
Smith Amanda W.,
Elbert Donald L.
Publication year - 2013
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201300023
Subject(s) - ethylene glycol , self healing hydrogels , peg ratio , chemical engineering , scaffold , aqueous solution , sodium dodecyl sulfate , chemistry , dextran , copolymer , polymer chemistry , porosity , microsphere , materials science , chromatography , biomedical engineering , organic chemistry , polymer , medicine , finance , engineering , economics
Clickable poly(ethylene glycol) (PEG) derivatives are used with two sequential aqueous two‐phase systems to produce microsphere‐based scaffolds for cell encapsulation. In the first step, sodium sulfate causes phase separation of the clickable PEG precursors and is followed by rapid geleation to form microspheres in the absence of organic solvent or surfactant. The microspheres are washed and then deswollen in dextran solutions in the presence of cells, producing tightly packed scaffolds that can be easily handled while also maintaining porosity. Endothelial cells included during microsphere scaffold formation show high viability. The clickable PEG‐microsphere‐based cell scaffolds open up new avenues for manipulating scaffold architecture as compared with simple bulk hydrogels.