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Non‐Natural Amino Acids for Protein Engineering and New Protein Chemistries
Author(s) -
Kwon Inchan,
Lim Sung In
Publication year - 2013
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201200710
Subject(s) - amino acid , bioconjugation , protein engineering , chemistry , sequence space , sequence (biology) , protein design , biochemistry , computational biology , protein structure , biology , enzyme , mathematics , pure mathematics , banach space
Non‐natural amino acids can be used to expand the protein sequence space significantly beyond the limits set by nature. Expanding the protein sequence space opens a new door to engineering and chemically modifying proteins. Reassigning codons to non‐natural amino acids as well as engineering protein translational machinery is required to incorporate non‐natural amino acids into a single or multiple sites of a target protein in cells. Non‐natural amino acid incorporation holds a great promise in substantially improving protein intrinsic properties and providing new orthogonal chemistries to proteins for bioconjugation.