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Synthesis of a Bifunctional Fluorescent Polymer for Cell Imaging and Enzyme Detection
Author(s) -
Wen Quanshan,
Zhu Chunlei,
Liu Libing,
Yang Qiong,
Wang Shu,
Zhu Daoben
Publication year - 2012
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201200419
Subject(s) - phenylboronic acid , bifunctional , polyfluorene , cationic polymerization , chemistry , conjugated system , polymer , side chain , fluorescence , polymer chemistry , moiety , combinatorial chemistry , live cell imaging , organic chemistry , cell , biochemistry , physics , quantum mechanics , catalysis
Abstract A new bifunctional cationic conjugated polyfluorene derivative (PFPBOH) containing phenylboronic acid group has been synthesized and characterized. The phenylfluorenyl backbone and positively charged groups in the side chain give the polymer PFPBOH excellent fluorescence property and good water solubility. Phenylboronic acid moieties on the side chain of the polymer form cyclic esters with adjacent diols on cell membrane, which can be employed in cell imaging. Besides that, the fluorescence of PFPBOH can be quenched by p‐nitroaniline released via enzyme reaction, thus, the polymer can be used for γ‐glutamyltranspeptidase detection through a fluorescence “turn off” mechanism. These findings exhibit great potential for developing multifunctional polymer materials for simultaneous imaging and detection.