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Oligoepoxide‐Based Monoliths: Synthesis and Application as Affinity Capillary Column for Enrichment of Immunoglobulin G
Author(s) -
Pecher Heike S.,
Zimathies Annett,
Weller Michael G.
Publication year - 2012
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/macp.201200206
Subject(s) - monolith , porosimetry , porosity , scanning electron microscope , polymerization , capillary action , chemistry , chromatography , chemical engineering , epoxide , polymer chemistry , vinyl ether , copolymer , materials science , polymer , porous medium , organic chemistry , catalysis , composite material , engineering
A series of new epoxide‐based monoliths is synthesized by self‐polymerization of polyglycerol polyglycidyl ether as porous support for affinity chromatography. Porosity and mean pore sizes are investigated as a function of the porogen ratio and analyzed by mercury porosimetry. The morphologies are visualized by environmental scanning electron microscope. With a particularly rigid monolith prepared with 75 vol% porogen (66% porosity and 12 μm mean pore size), a capillary column (0.5 mm ID) is prepared and directly functionalized with recombinant protein A to extract immunoglobulin G from rabbit serum.